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熒光素酶

·  英文名稱 : Luciferase

·  CAS : 61970-00-1

·  儲存條件 : -20℃

recombinant, expressed in E. coli, buffered aqueous solution, ≥10×10^10 units/mg protein

Unit Definition

One luciferase enzyme unit will produce one Relative Light Unit (RLU) at 20-25 °C over a 10 second period, measured in 100 μl assay mixture containing 40 pmol ATP and 15 nmol luciferin in Tris-glycine buffer, pH 7.6, using a GloMax 20/20 Luminometer.
Unit Definition Conversion Factor: There are approximay 9000 Relative Light Units (RLU) per one traditional Light Unit that uses a peak height equivalent to 0.02 μCi of 14C in a PPO/POPOP cocktail.
Physical form

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Supplied a a solution in 25mM Tris-acetate, pH 7.8 , 1mM EDTA, 1mM DTT, 0.2M ammonium sulfate, 15% glycerol and 30% ethylene glycol

recombinant   expressed in E. coli
assay   ≥98% (SDS-PAGE)
form   buffered aqueous solution

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