重組小鼠巨噬細(xì)胞集落刺激因子
M-CSF 也稱為 CSF-1，是一種四α螺旋束細(xì)胞因子，是巨噬細(xì)胞存活、增殖和分化的主要調(diào)節(jié)劑 。M-CSF 蛋白對(duì)于破骨細(xì)胞祖細(xì)胞的存活和增殖也至關(guān)重要。M-CSF 還可啟動(dòng)和增強(qiáng)巨噬細(xì)胞對(duì)腫瘤細(xì)胞和微生物的殺傷，調(diào)節(jié)巨噬細(xì)胞釋放細(xì)胞因子和其他炎癥調(diào)節(jié)劑，并刺激胞飲作用 。
(純度) Purity
≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
(內(nèi)毒素) Endotoxin
≤10 EU/mg by the LAL method.
(生物活性) Bioactivity
The activity is determined by the dose-dependent proliferation of murine NFS-60 cells is ＜ 0.05 ng/mL, corresponding to a specific activity of ≥ 2 E7 units/mg.
(種屬) Species
Mouse
(表達(dá)系統(tǒng)) Expression System
CHO
(預(yù)測(cè)分子量大小) Mol. Weight
26 kDa (monomer)
(表達(dá)區(qū)間) Expression Region
Lys33-Glu262
(標(biāo)簽) Tag Info
Tag free
(劑型) Formulation
Lyophilized
(溶液) Buffer
Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% Mannitol and 0.01% Tween 80, pH7.4.
(規(guī)格) Size：1mg
(復(fù)溶) Reconstitution
It is recommended to redissolve in sterile deionized water.
(運(yùn)輸) Shipping
The product is shipped with blue ice.
(存儲(chǔ)) Storage & Stability
36 months at -20°C to -80°C in lyophilized state;
6 months at -20°C to -80°C under sterile conditions after reconstitution;
7-10 days at 2°C to 8°C under sterile conditions after reconstitution;
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.?
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